pksJ [2018-02-09T20:33:44.000Z]

polyketide synthase

locus: BSU17180

pI: 5.00

mw: 562.00 kDa

protein length: 5045 aa

gene length: 15135 bp

function: polyketide synthesis

product: polyketide synthase

essential: no

synonyms: pksK

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.1|Biosynthesis of antibacterial compounds]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.15|Biosynthesis of antibacterial compounds]

Gene

Coordinates: 1,792,806 → 1,807,937

The protein

Protein family

• ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)

Paralogous protein(s)

• shares highly conserved domains of more than 1,000 amino acids with: [protein|95B75CFF126AF0AD845E62B4036DD3B6DAC8C7FA|DhbF], [protein|13EB6B903088C11D985DDB7CEC864797058FE038|PksJ], [protein|66AFF1FC7D1BBA8EA5BB57A935FF5AB890A895C1|PksN], [protein|E3D7EF8F165CAC2682B0D620081C5C6E555E43C4|PpsA], [protein|E7A2F93B33B1899313893D362D92405232BC9097|PpsB], [protein|D11CE89E2AC2ADE3FEFEC261E295EC5906CE6407|PpsC], [protein|E73D9F1A8F54DAD4B2056CABBE580D7AD36CB042|PpsD], [protein|C1C051209943E02369356075EAA733297E9478CB|PpsE], [protein|81845F44CE2C601555066E31E87384FF5D7B4139|SrfAA], [protein|3C484FDB3A440269E87D6B6E8500A875AC456666|SrfAB], [protein|1D5F227860A321506A1AB4BAE63CAD3A66E43BFC|SrfAC]

Structure

• [PDB|4NA1] [Pubmed|24508341]

Expression and Regulation

Operons

• genes: [gene|55C821F68B2E5F9F45CC2B2C9494D48D5C33266A|pksC]-[gene|F3E9CA9330394FD829D59B0C2D4D1EEDD2C22D3B|pksD]-[gene|CB0B856B22984DD5B10F01DD07C3961FAA742923|pksE]-[gene|6BEC9E2BCECE33803EAC14DE7E8E988FC9C8DC8B|acpK]-[gene|85D6E439529E8B3F73244A0B004B394B4FE65509|pksF]-[gene|F4C96980640CB04D3017B8EBB02918006CBCF7BB|pksG]-[gene|A4CF68F602273CFDFD55EFD2C1164F0E2E8F6972|pksH]-[gene|F437A90A97CF8ED4BA1E5CCA8A33D9E93612902B|pksI]-[gene|13EB6B903088C11D985DDB7CEC864797058FE038|pksJ]-[gene|BABBC5848F9BBF9E9318252F5AD7D1C4F55FCEAB|pksL]-[gene|40779D3A6E4F72EEFD702BF9FA15B57951783D12|pksM]-[gene|66AFF1FC7D1BBA8EA5BB57A935FF5AB890A895C1|pksN]-[gene|85213235F7D3D8E711963E53F314ED758B5FA94B|pksR]
  description: [Pubmed|22383849]

  regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|24187085], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]
  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: activation, [Pubmed|24187085], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]

  regulation

  • expressed during the transition from growth to stationary phase ([protein|search|AbrB], [protein|search|CodY]) [Pubmed|24187085]

  additional information

  • this is a very large operon comprising about 75 kb

• genes: [gene|647B1D7E496E81E7FC7FD2BDCACC2486852BAC19|pksB]-[gene|55C821F68B2E5F9F45CC2B2C9494D48D5C33266A|pksC]-[gene|F3E9CA9330394FD829D59B0C2D4D1EEDD2C22D3B|pksD]-[gene|CB0B856B22984DD5B10F01DD07C3961FAA742923|pksE]-[gene|6BEC9E2BCECE33803EAC14DE7E8E988FC9C8DC8B|acpK]-[gene|85D6E439529E8B3F73244A0B004B394B4FE65509|pksF]-[gene|F4C96980640CB04D3017B8EBB02918006CBCF7BB|pksG]-[gene|A4CF68F602273CFDFD55EFD2C1164F0E2E8F6972|pksH]-[gene|F437A90A97CF8ED4BA1E5CCA8A33D9E93612902B|pksI]-[gene|13EB6B903088C11D985DDB7CEC864797058FE038|pksJ]-[gene|BABBC5848F9BBF9E9318252F5AD7D1C4F55FCEAB|pksL]-[gene|40779D3A6E4F72EEFD702BF9FA15B57951783D12|pksM]-[gene|66AFF1FC7D1BBA8EA5BB57A935FF5AB890A895C1|pksN]-[gene|85213235F7D3D8E711963E53F314ED758B5FA94B|pksR]
  description: [pubmed|22383849]

Biological materials

Mutant

• BKE17180 (Δ[gene|13EB6B903088C11D985DDB7CEC864797058FE038|pksJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE17180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACGCTGCCTCCATTTC, downstream forward: _UP4_ACTGCGGCAAACATATGGGA
• BKK17180 (Δ[gene|13EB6B903088C11D985DDB7CEC864797058FE038|pksJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK17180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACGCTGCCTCCATTTC, downstream forward: _UP4_ACTGCGGCAAACATATGGGA

References

• 17190806,22383849,24187085,24508341